24 research outputs found

    Chemical composition and biological activities assessment of olive fruit volatiles from different varieties grown in Tunisia

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    Volatile compounds, present in olives, are responsible for the olive fruit flavor and oil aroma, influencing the consumer’s preference. These compounds have a biological activity to fight off pathogens. The aim of this work is to characterize volatiles in pulps and cores of Chemlali, Arbequina and Koroneiki olives, collected from Menzel Mhiri-Kairouan, and to test both the efficiency of these compounds against two bacteria and six phytopathogenic fungal species, by diffusion and dilution methods, and their antioxidants activities. The analyzis of volatiles were determined by GC-FID and GC-MS in three cultivars at the full ripening stage. Thirty five compounds were identified, such us an assortment of phenol, alcohol, hydrocarbon, aldehyde and terpenes derivatives. A high changes in volatiles was noted between cultivars and fruit organs. In fact, the major compounds in the pulps and the cores, of different cultivars, are (E)-2-decenal (46.9%), nonanal (19.6%), 1-hexadecene (16.3%), 7-methyl-1,3,5-cycloheptatriene 7-methyl-1,3,5-cycloheptatriene (15.47%), (E,E)-2,4-decadienal (14.5%) and 1-tetradecene (14.6%). Also, the cores volatiles illustrated more richness in aldehydes than the pulps for all cultivars. Volatile fractions exhibited a moderate to important antibacterial activities against bacteria. However, Arbequina cores volatiles and both Chemlali and Koroneiki pulps volatiles established a moderate to higher activities against tested fungi. The DPPH and ABTS˙âș tests demonstrated that the highest antioxidant capacity of volatiles were assigned to Arbequina cores and Koroneiki pulps. The Principal Components Analysis showed a significant relationship between antioxidants and/or antimicrobial properties and the levels of the main volatile compounds (limonene, methyldecane, nonanal, E-2-decenal, camphor, geranic acid, tetradecene, hexadecane, tetradecane) in different fruit organs

    Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato

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    Five endophytic Bacillus spp. isolated from surface-sterilized stem tissues of wild Solanaceous species, i.e. Datura metel, Solanum nigrum and S. elaeagnifolium, were screened for their in vitro and in vivo antagonistic potential against Fusarium oxysporum f. sp. lycopersici (FOL). Pathogen sporulation was totally suppressed by these five strains and its mycelial growth was significantly decreased using bacterial whole-cell suspensions and cell-free culture filtrates. Extracellular metabolites remained effective after heating at 50–100 °C with a decline in activity with treatments involving temperatures at 100 °C, proteinase K addition, and pH adjustments to 2 or 12. Chitinase and lipopeptide antibiotic genes were detected using PCR amplification. The five strains produced salicylic acid when grown in succinate medium, with the greatest production exhibited by B. subtilis str. SV41. In planta application of Bacillus spp. cell-free culture filtrates and whole-cell suspensions significantly decreased Fusarium wilt severity by 87–100% and enhanced tomato growth by 38–80%, compared to FOL-inoculated and untreated controls. The two Bacillus strains exhibiting the strongest decrease in Fusarium wilt severity (B. subtilis str. SV41 and B. amyloliquefaciens subsp. plantarum str. SV65) were further evaluated for their ability to induce systemic resistance (ISR) in FOL-infected and uninfected tomato plants. Using quantitative RT-PCR, the expression of the acidic PR-1 and PR-3 genes as well as the lipoxygenase (LOXD) gene were significantly induced in plants treated with B. amyloliquefaciens subsp. plantarum str. SV65. This effect was observed in both FOL-inoculated and uninoculated plants. © 201

    Contact effects in high performance fully printed p-channel organic thin film transistors

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    Contact effects have been investigated in fully printed p-channel organic thin film transistors with field effect mobility up to 2 cm2/Vs. Electrical characteristics of the organic thin film transistors, with channel lengt
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